RNA

Part:BBa_M36131:Design

Designed by: Vir Choksi, Daniel McHugh   Group: Stanford BIOE44 - S11   (2012-06-01)

2D RNA scaffold


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 71
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 71
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 71
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 71
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 71
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We placed d2' and the PP7 aptamer under a rhamnose inducible promoter (not included here). d2" and the MS2 aptamer are under the control of another rhamnose inducible promoter (included here).

Source

Delebecque et al., Science 2011

References